IF Protocol Hub

Editorial policy

The sourcing, review, image, authorship, versioning, and correction standards used by IF Protocol Hub.

Evidence and scope

Scientific claims should be traceable to primary literature, established methods references, manufacturer documentation, or clearly identified laboratory experience. General principles are separated from sample-, antibody-, and instrument-specific conditions whenever possible.

Protocol language

Conditions are presented as starting points or comparison variables unless a source supports a more specific recommendation. Bright signal alone is not treated as evidence of specificity; controls, expected localization, morphology, and acquisition settings are considered together.

Images and illustrations

Original educational illustrations may be digitally generated or edited. They are used to explain workflows, optical relationships, controls, and artifact patterns, and are not presented as experimental data. Third-party figures are not used without an appropriate license, permission, or clear attribution.

Review claims

The site does not describe a page as scientifically reviewed or verified unless a defined review has been completed. Readers should treat all protocols as guidance that requires local validation with the actual specimen, antibody, and microscope.

Corrections and revisions

Material changes that affect scientific meaning, interpretation, safety, or cited evidence are recorded on the corrections page. Suggestions and error reports can be submitted through the contact page.