Decision guides
Understand which variable you are changing and why.
A protocol tells you what happened. A decision guide helps you predict what may change when the specimen, target, antibody, or instrument changes.
Preparation
Fixation
Balance structure preservation and epitope accessibility.
Open guide → AccessPermeabilization
Choose access according to target compartment and membrane preservation.
Open guide → Focused comparisonSimultaneous fixation and permeabilization
Evaluate combined paraformaldehyde and Triton X-100 exposure against a sequential workflow.
Open guide → FFPEAntigen retrieval
Treat retrieval as an evidence-based optimization problem.
Open guide → EvidenceControls
Distinguish technical background, specificity, expression, and imaging artifacts.
Open guide → ImagingFluorophore selection
Match channels to target abundance and specimen background.
Open guide → DiagnosisTroubleshooting
Start from the observed pattern and run the smallest discriminating test.
Open guide →